Influence of upper body stretching exercises on shot put / Influência dos exercícios de alongamento da parte superior do corpo no arremesso de peso / Influencia de los ejercicios de estiramiento de la parte superior del cuerpo en el lanzamiento de peso

ABSTRACT Introduction The technique in the shot put and the ability in the throwing stage are two important factors that determine the ability of athletes. Qualified experts and coaches attach great importance to training and research on the ability of the throwing stage and the ability to throw. Objective Compare gravity load training and single incremental load training through practical means, analyzing the impacts on throwing ability in athletes. Methods The self-assessment method was used to conduct the comparative experiment on different forms of strength training in 20 college students. The experimental scheme adopts the single incremental load strength training, and the control scheme adopts the traditional barbell training. Results : After the experiment, the hand angle of group I was significantly higher than that of group II (P < 0.05); after the experiment, the shoulder angle of group I was significantly higher than that of group II (P < 0.05); after the experiment, the performance of the seated shot placed in group I was significantly higher than that of group II (P < 0.05), and there was no significant difference in the standing performance (P > 0.05). Conclusion Single incremental load training can significantly improve the throwing ability of shot put athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução A técnica no arremesso de peso e a habilidade na etapa de arremessar são dois fatores importantes que determinam a habilidade dos atletas Especialistas e treinadores qualificados atribuem grande importância ao treinamento e pesquisa sobre a habilidade da fase de arremesso e a capacidade de arremessar. Objetivo Comparar o treinamento com carga gravitacional e o treinamento com carga incremental única através de meios experimentais, analisando os impactos na habilidade de arremesso nos atletas. Métodos O método de auto-avaliação foi usado para realizar o experimento comparativo de diferentes formas de treinamento de força em 20 estudantes universitários. O esquema experimental adota o treinamento único de força de carga incremental, e o esquema de controle adota o treinamento tradicional de barra. Resultados : Após o experimento, o ângulo da mão do grupo I foi significativamente maior do que o do grupo II (P < 0,05); após o experimento, o ângulo do ombro do grupo I foi significativamente maior do que o do grupo II (P < 0,05); após o experimento, o desempenho do tiro sentado colocado no grupo I foi significativamente maior do que o do grupo II (P < 0,05), e não houve diferença significativa no desempenho em pé (P > 0,05). Conclusão O treinamento com carga incremental única pode melhorar significativamente a capacidade de arremesso dos atletas de arremesso de peso. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción La técnica en el lanzamiento de peso y la habilidad en la fase de lanzamiento son dos factores importantes que determinan la habilidad de los atletas. Los especialistas y entrenadores cualificados atribuyen gran importancia a la formación y a la investigación sobre la habilidad de la fase de lanzamiento y la habilidad de lanzamiento. Objetivo Comparar el entrenamiento con carga gravitacional y el entrenamiento con carga incremental única a través de medios experimentales, analizando los impactos en la capacidad de lanzamiento en los atletas. Métodos Se utilizó el método de autoevaluación para realizar el experimento comparativo de diferentes formas de entrenamiento de fuerza en 20 estudiantes universitarios. El esquema experimental adopta el entrenamiento de fuerza con una sola carga incremental, y el esquema de control adopta el entrenamiento tradicional con barra. Resultados : Después del experimento, el ángulo de la mano del grupo I fue significativamente mayor que el del grupo II (P < 0,05); después del experimento, el ángulo del hombro del grupo I fue significativamente mayor que el del grupo II (P < 0,05); después del experimento, el rendimiento del lanzamiento de peso sentado del grupo I fue significativamente mayor que el del grupo II (P < 0,05), y no hubo diferencias significativas en el rendimiento de pie (P > 0,05). Conclusión El entrenamiento con una sola carga incremental puede mejorar significativamente la capacidad de lanzamiento de los atletas de lanzamiento de peso. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Hypothermic Circulatory Arrest in Median Sternotomy Hemorrhage During Redo Aortic Surgery

ABSTRACT Introduction: This study summarizes the clinical data of patients who developed sternotomy hemorrhage during redo aortic surgery and analyzes the clinical experience of using hypothermic circulatory arrest. Methods: We retrospectively analyzed the medical records of patients who developed sternotomy hemorrhage during redo aortic surgery from May 2018 to August 2021. General anesthesia with single-lumen tracheal intubation was used. Femoral artery, vein, and superior vena cava cannulation were used if cardiopulmonary bypass was required according to the situation, and right superior vein or apical cannulation was selected for left heart drainage. Results: A total of 11 patients were enrolled in this study, comprising nine males and two females, with an average age of 44.3±16.7 years. All cases were successfully completed without cerebrovascular complications or paraplegia. Two patients died during hospitalization, two patients died during the follow-up after discharge, and the remaining patients are recovering well. Conclusion: The femoral-femoral bypass with hypothermic circulatory arrest technique is a safe and reliable method to use in cases of sternotomy hemorrhage during redo aortic surgery.

Impacts of aerobic exercise on the physical fitness of shot put athletes / Impactos do exercício aeróbico na aptidão física dos atletas de arremesso / Efectos del ejercicio aeróbico en la aptitud física de los atletas de lanzamiento

ABSTRACT Introduction: Shot put is a competitive sport that combines speed, strength, and explosiveness. Training the skill with aerobic exercise has attracted the attention of many coaches and scholars on competitive sports training. Shot put is a fast-powered activity, and its shooting speed is the determining factor for sports performance. It is believed that aerobic exercise can improve the ability of its practitioners. Objective: To analyze the impacts of aerobic exercise on the physical fitness of shot put athletes. Methods: 18 shot put athletes were randomly selected as volunteers for research that consisted of a random division between experimental and control groups. The experimental group received a protocol involving aerobic training, while the control group received regular training before, during, and after the experiment. The maximal strength and explosive power of the two groups of athletes were tested before and after the intervention, and mathematical-statistical methods were used to process the collected data. Results: The maximum strength index of both groups was improved; however, the improvement in the experimental group was statistically relevant(P<0.05). The values of the pressure index, power, and balance of the athletes from the experimental group were statistically altered to those before the experiment (P<0.01). Conclusion: The implementation of aerobic exercise in the routine of shot put athletes can effectively improve the performance in the sport and the special abilities of its practitioners. The training method analyzed was also shown to improve specific performance in the shot put athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O arremesso é um esporte de competição que combina velocidade, força e explosividade. O treinamento da capacidade com exercício aeróbico atraiu a atenção de muitos treinadores e estudiosos sobre treinamento esportivo de competição. O arremesso é uma atividade de potência rápida, e sua velocidade de tiro é o fator determinante para o desempenho esportivo. Acredita-se que o exercício aeróbico possa melhorar a habilidade de seus praticantes. Objetivo: Analisar os impactos do exercício aeróbico na aptidão física dos atletas de arremesso. Métodos: 18 praticantes de arremesso foram selecionados aleatoriamente como voluntários para uma pesquisa que consistiu na divisão aleatória entre grupos experimental e de controle. O grupo experimental recebeu um protocolo envolvendo o treinamento aeróbico, enquanto o grupo de controle recebeu seu treinamento regular antes, durante e depois do experimento. A força máxima e o poder explosivo dos dois grupos de atletas foram testados antes e após a intervenção, foram usados métodos estatísticos matemáticos para processar os dados coletados. Resultados: O índice de força máxima dos dois grupos foi aprimorado, porém a melhoria no grupo experimental foi estatisticamente relevante(P<0,05). Os valores do índice de pressão, potência e balanço dos atletas do grupo experimental foram estatisticamente alterados em relação aqueles anteriores ao experimento (P<0,01). Conclusão: A implementação do exercício aeróbico na rotina dos atletas de arremesso pode efetivamente melhorar o desempenho no esporte e as habilidades especiais dos seus praticantes. O método de treinamento analisado também demonstrou melhorar o desempenho específico nos esportistas de arremesso. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

Resumen Introducción: El lanzamiento es un deporte de competición que combina velocidad, fuerza y explosividad. El entrenamiento de la capacidad con ejercicios aeróbicos ha atraído la atención de muchos entrenadores y estudiosos del entrenamiento deportivo de competición. El lanzamiento es una actividad de potencia rápida, y su velocidad de tiro es el factor determinante para el rendimiento deportivo. Se cree que el ejercicio aeróbico puede mejorar la capacidad de sus practicantes. Objetivo: Analizar los impactos del ejercicio aeróbico en la condición física de los atletas de lanzamiento. Métodos: Se seleccionaron aleatoriamente 18 practicantes de lanzamiento como voluntarios para una investigación que consistió en la división aleatoria entre los grupos experimental y de control. El grupo experimental recibió un protocolo de entrenamiento aeróbico, mientras que el grupo de control recibió su entrenamiento habitual antes, durante y después del experimento. Se comprobó la fuerza máxima y la potencia explosiva de los dos grupos de atletas antes y después de la intervención, y se utilizaron métodos estadísticos matemáticos para procesar los datos recogidos. Resultados: El índice de fuerza máxima de ambos grupos mejoró, aunque la mejora en el grupo experimental fue estadísticamente relevante (P<0,05). Los valores del índice de presión, la potencia y el equilibrio de los atletas del grupo experimental estaban estadísticamente alterados en relación con los anteriores al experimento (P<0,01). Conclusión: La implementación del ejercicio aeróbico en la rutina de los atletas de lanzamiento puede mejorar efectivamente el rendimiento en el deporte y las habilidades especiales de sus practicantes. El método de entrenamiento analizado también ha demostrado mejorar el rendimiento específico de los atletas de lanzamiento. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Research on the causes of sports injuries and rehabilitation in university physical training / Pesquisa sobre as causas de lesões esportivas e reabilitação no treinamento físico universitário / Investigación sobre las causas de lesiones deportivas y rehabilitación en el entrenamiento físico universitario

ABSTRACT Introduction College students represent a large proportion of amateur athletes in China. In sports practices, some unavoidable situations lead to physical injuries that can seriously affect the daily life of these students. Formulating rehabilitation plans to avoid secondary complications and speed up the students' return to their daily activities is necessary. Objective To study the causes of sports injuries and the effect of sports rehabilitation on university physical training. Methods Through questionnaires and interviews, 720 students and 25 teachers with a history of sports injuries were selected to investigate the causes of students' sports injuries. Then, 20 volunteers were selected as research subjects. They received a rehabilitation training protocol three times a week for six weeks. EMG signals of the athletes' shoulder and neck muscles were measured. Results Sports injury is an inevitable problem during college physical training. Training joint flexibility and stability can effectively improve the surface EMG signals in the area adjacent to the joint to improve muscle strength and joint amplitude level. Conclusion Students should consciously undertake rehabilitation training under professional guidance and receive full instruction in sports rehabilitation procedures involving a combination of physical and psychological recovery. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução Estudantes universitários representam grande parte dos esportistas amadores na China. Nas práticas esportivas, algumas situações inevitáveis acarretam em lesões físicas que podem afetar seriamente o cotidiano desses alunos. Faz-se necessária a formulação de planos de reabilitação afim de evitar complicações secundárias e agilizar o retorno dos estudantes às suas atividades diárias. Objetivo Estudar as causas de lesões esportivas e o efeito da reabilitação esportiva no treinamento físico universitário. Métodos 720 alunos e 25 professores com histórico de lesão esportiva foram selecionados para investigar as causas de lesão esportiva dos alunos por meio de questionário e entrevista. Em seguida, foram selecionados 20 voluntários como objeto de pesquisa. Esses receberam um protocolo de treinamento em reabilitação três vezes por semana, durante 6 semanas. Foram mensurados os sinais EMG dos músculos do ombro e pescoço dos atletas. Resultados A lesão esportiva é um problema inevitável durante o treinamento físico universitário. Treinar a flexibilidade e estabilidade articular pode efetivamente melhorar os sinais EMG de superfície na área adjacente à articulação, de modo a melhorar a força muscular e o nível de amplitude articular. Conclusão Os alunos devem realizar conscientemente treinamento de reabilitação sob orientação profissional, além de receberem instrução plena sobre os procedimentos da reabilitação esportiva, envolvendo uma combinação de recuperação física e psicológica. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Los estudiantes universitarios representan una gran parte de los deportistas aficionados en China. En las prácticas deportivas, algunas situaciones inevitables provocan lesiones físicas que pueden afectar gravemente a la vida cotidiana de estos alumnos. Es necesario formular planes de rehabilitación para evitar complicaciones secundarias y acelerar el regreso de los alumnos a sus actividades cotidianas. Objetivo Estudiar las causas de las lesiones deportivas y el efecto de la rehabilitación deportiva en el entrenamiento físico universitario. Métodos Se seleccionaron 720 estudiantes y 25 profesores con antecedentes de lesiones deportivas para investigar las causas de las lesiones deportivas de los estudiantes mediante un cuestionario y una entrevista. A continuación, se seleccionaron 20 voluntarios como sujetos de la investigación. Recibieron un protocolo de entrenamiento de rehabilitación tres veces por semana, durante 6 semanas. Se midieron las señales EMG de los músculos del hombro y del cuello de los atletas. Resultados Las lesiones deportivas son un problema inevitable durante el entrenamiento físico universitario. El entrenamiento de la flexibilidad y la estabilidad articulares pueden mejorar eficazmente las señales EMG superficiales en la zona adyacente a la articulación, de modo que se mejore la fuerza muscular y el nivel de amplitud articular. Conclusión Los estudiantes deben realizar conscientemente un entrenamiento de rehabilitación bajo la dirección de un profesional, y recibir una instrucción completa en los procedimientos de rehabilitación deportiva que impliquen una combinación de recuperación física y psicológica. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Correlation between selenium and chronic kidney disease: a meta-analysis / 预防医学

Objective@#To systematically evaluate the correlation between selenium in the body and the risk of chronic kidney disease (CKD), so as to provide insights into CKD prevention and control.@*Methods@#Publications pertaining to the correlation between selenium level and CKD were retrieved from electronic databases, including CNKI, WanFang Data, PubMed and Web of Science from inception until July 28, 2022. Meta-analysis was performed using the software Stata 16.0, and all measures were expressed with standardized mean difference (SMD) and its 95%CI. The source of heterogeneity was analyzed using subgroup analysis and meta-regression, and sensitivity analysis was performed using the leave-one-out method. In addition, the publication bias was evaluated with d funnel plot, Egger's test and Begg's test, and the robustness of the result was evaluated using the trim and filling method.@*Results@#A total of 2 990 publications were screened, and 63 eligible publications were included in the final analysis, including 18 cross-sectional studies, 40 case-control studies, 2 cohort studies and 3 randomized controlled trials (RCTs), which covered 5 099 CKD patients and 6 334 controls. Meta-analysis showed lower selenium levels in CKD patients than in controls (SMD=-1.828, 95%CI: -2.132 to -1.523, P<0.001). Subgroup analysis showed lower selenium levels among CKD patients than among controls from Asia, Europe and multiple continents (P<0.05), and lower selenium levels were detected in patients undergoing conservative treatment, hemodialysis and peritoneal dialysis than in controls (P<0.05), while the selenium levels were significantly lower in CKD patients than in controls in cross-sectional studies, case-control studies and RCTs (P<0.05). The selenium level was significantly lower among CKD patients than among controls regardless of the study year, sample size and score for quality of publications (P<0.05). Sensitivity analysis showed robustness of this meta-analysis. Publication bias was identified by funnel plot, Begg's test and Egger's test, and the robustness of the result was found using the trim-and-fill method. @*Conclusion @#There may be a possible correlation between selenium and the risk of CKD.

Research progress on role of free fatty acid receptors in nervous system diseases / 中国生物制品学杂志

@#Free fatty acid receptors(FFARs)are a series of orphan G protein-coupled receptors(GPCRs)activated by free fatty acids(FFAs)and their derivatives. As transmembrane receptors,GPCRs are involved in the occurrence and development of many diseases and provide a wide range of therapeutic targets for these diseases. FFARs combined with FFAs are mainly involved in the secretion of endocrine hormones such as insulin,adipocyte differentiation,inflammatory response,autoimmune response and other processes,which was a potential therapeutic target for energy metabolism disorders and immune diseases. However,recent studies have shown that FFAs and its receptor FFARs are widely involved in neuroinflammation and neuroimmunity directly or through the brain-intestinal axis,and are expected to be a therapeutic target for multiple sclerosis,Alzheimer′s disease(AD),Parkinson′s disease(PD),depression and other diseases. This paper reviews the research progress of the role of FFARs in nervous system diseases.

Lycopene inhibits proliferation and promotes apoptosis of renal cancer 786-O cells through the SIRT1/NF-κB axis / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨番茄红素通过沉默信息调节因子1（SIRT1）/核因子-κB（NF-κB）轴对肾癌786-O细胞增殖、凋亡的影响。方法：常规培养人正常肾细胞HK-2和人肾癌细胞786-O，实验分为对照组（0.1% DMSO）、顺铂组（40 μg/mL）、番茄红素低质量浓度（2.5 μg/mL）组、番茄红素高质量浓度（5 μg/mL）组、番茄红素（5 μg/mL）+EX527（SIRT1抑制剂）（3 µmol/L）组。CCK-8法、克隆形成实验检测各组HK-2、786-O细胞的增殖能力，流式细胞术检测各组786-O细胞的凋亡，RH123、DCFH-DA染色分别检测各组786-O细胞的线粒体膜电位（MMP）、活性氧（ROS）水平，WB法检测各组786-O细胞中凋亡相关蛋白BAX、Bcl-2、C-casp3和SIRT1/NF-κB轴相关蛋白SIRT1、p-NF-κB蛋白的表达。786-O细胞移植瘤实验检测番茄红素低（5 mg/kg）、高质量浓度（20 mg/Kg）、顺铂（2 mg/kg）、番茄红素（20 mg/kg）+EX527（10 mg/kg）对移植瘤生长的影响，TUNEL法检测各组移植瘤组织中的细胞凋亡。结果：番茄红素呈剂量依赖性地抑制786-O细胞的增殖活性，番茄红素、顺铂均明显抑制786-O细胞的克隆形成能力且促进其凋亡，细胞中MMP损伤率升高而ROS水平降低，凋亡相关蛋白BAX、C-casp3表达均显著升高（均P<0.05）而Bcl-2表达下调（P<0.05），SIRT1表达显著升高（P<0.05）而p-NF-κB的表达显著降低（P<0.05），上述作用均可被EX527逆转；番茄红素、顺铂抑制786-O细胞移植瘤的生长且促进其细胞凋亡，其作用也能被EX527逆转。结论：番茄红素通过上调SIRT1、抑制NF-κB通路的激活进而抑制786-O细胞增殖且诱导其凋亡。

Diagnostic and prognostic significance of long noncoding RNA LINC00173 in patients with melanoma

SUMMARY OBJECTIVE: A growing volume of literature has suggested long noncoding RNAs (lncRNAs) as important players in tumor progression. In this study, we aimed to investigate the expression and prognostic value of lncRNA LINC00173 (LINC00173) in melanoma. METHODS: LINC00173 expression was measured in 163 paired cancerous and noncancerous specimen samples by real-time polymerase chain reaction. The correlations between LINC00173 expression with clinicopathological characteristics and prognosis were analyzed by chi-square test, log-rank test, and multivariate survival analysis. Receiver-operating characteristic curves were used for the assessment of the diagnostic value of LINC00173 for melanoma patients. RESULTS: The expression level of LINC00173 in melanoma specimens was distinctly higher than that in adjacent non-neoplasm specimens (p<0.01). Besides, LINC00173 was expressed more frequently in patients with advanced melanoma than in patients with early melanoma. Multivariate assays confirmed that LINC00173 expression level was an independent prognostic predictor of melanoma patients (p<0.05). CONCLUSION: Our data indicated that LINC00173 expression could serve as an unfavorable prognostic biomarker for melanoma patients.

Lactic acidosis associated with standard dose linezolid in a kidney recipient with impaired renal function

Abstract Severe lactic acidosis, a mitochondrial toxicity caused by the recommended standard dosage of linezolid (LZD), may occur in patients with impaired renal function. We describe an adult male who underwent kidney transplantation with stably impaired renal function, severe dyspnea, and abdominal discomfort. He received a standard oral dose of LZD (600 mg twice daily) and azithromycin for three weeks with a reduced immunosuppressant dose due to pulmonary non-tuberculosis mycobacterial infection. He was alert and afebrile, with a blood pressure of 140/60 mmHg. Pertinent laboratory data showed: pH 7.12, PaCO2 13.6 mmHg; HCO3- 4.3 mmol/L and serum lactate 18.4 mmol/L. His trough serum LZD concentration reached toxic levels (21.4 μg/mL). With hemodialysis, his clinical symptoms improved, with a decline in serum LZD (9.8μg/mL) and lactate (3.2 mmol/L). Chronic standard dose LZD in patients with impaired renal function can lead to life-threatening lactic acidosis, especially in coexisting conditions that reduce LZD metabolism.

Tongue image segmentation and tongue color classification based on deep learning / 数字中医药（英文）

@#Objective To propose two novel methods based on deep learning for computer-aided tongue diagnosis, including tongue image segmentation and tongue color classification, improving their diagnostic accuracy. Methods LabelMe was used to label the tongue mask and Snake model to optimize the labeling results. A new dataset was constructed for tongue image segmentation. Tongue color was marked to build a classified dataset for network training. In this research, the Inception + Atrous Spatial Pyramid Pooling (ASPP) + UNet (IAUNet) method was proposed for tongue image segmentation, based on the existing UNet, Inception, and atrous convolution. Moreover, the Tongue Color Classification Net (TCCNet) was constructed with reference to ResNet, Inception, and Triple-Loss. Several important measurement indexes were selected to evaluate and compare the effects of the novel and existing methods for tongue segmentation and tongue color classification. IAUNet was compared with existing mainstream methods such as UNet and DeepLabV3+ for tongue segmentation. TCCNet for tongue color classification was compared with VGG16 and GoogLeNet. Results IAUNet can accurately segment the tongue from original images. The results showed that the Mean Intersection over Union (MIoU) of IAUNet reached 96.30%, and its Mean Pixel Accuracy (MPA), mean Average Precision (mAP), F1-Score, G-Score, and Area Under Curve (AUC) reached 97.86%, 99.18%, 96.71%, 96.82%, and 99.71%, respectively, suggesting IAUNet produced better segmentation than other methods, with fewer parameters. Triplet-Loss was applied in the proposed TCCNet to separate different embedded colors. The experiment yielded ideal results, with F1-Score and mAP of the TCCNet reached 88.86% and 93.49%, respectively. Conclusion IAUNet based on deep learning for tongue segmentation is better than traditional ones. IAUNet can not only produce ideal tongue segmentation, but have better effects than those of PSPNet, SegNet, UNet, and DeepLabV3+, the traditional networks. As for tongue color classification, the proposed network, TCCNet, had better F1-Score and mAP values as compared with other neural networks such as VGG16 and GoogLeNet.

Effect of reproductive health education on self efficacy and intention of condom use among college freshmen / 中国学校卫生

Objective@#To analyze the influence of reproductive health education in middle school on self efficacy and intention of condom use among college freshmen, so as to provide evidence for fertility protection related intervention and policy making.@*Methods@#A questionnaire survey was conducted among college freshmen in Beijing by hierarchical cluster sampling method. A total of 3 001 students were surveyed. The difference was compared by using χ 2 test and ANOVA. Multivariate linear and Logistic regression model was used to analyze the influencing factors associated with condom use self efficacy and condom use intention.@* Results@#The overall rate that reproductive health education received before college among freshmen in Beijing was 65.11%. The college freshmen from urban areas reported more adequate reproductive health education (71.11%) than those from non urban areas before college (59.36%)( P <0.05). The total scores of UNGASS (4.22±0.90 vs 4.05±0.98), condom use self efficacy (24.64±5.34 vs 23.09±4.93) and the intention of condom use (82.44% vs 70.88%) of college freshmen received reproductive health education in middle school were higher than those of college students without pre college reproductive health education( P <0.01). Multiple linear regression analysis showed that the score of condom use self efficacy of college students received pre college reproductive health education was higher than those without pre college reproductive health education( β =1.21, 95% CI =0.79-1.63, P <0.01). Multivariate Logistic regression analysis showed that reproductive health, the intention of condom use of college freshmen who received pre college reproductive health education was higher than that of college freshmen without reproductive health education( OR =1.63, 95% CI =1.33-2.01, P <0.01).@*Conclusion@#Reproductive health education in middle school can improve college freshmen s self efficacy and intention of condom use, contribute to the implementation of safe sex, and is of great significance to the protection of college students fertility.

Relationship between oxytocin receptor (OXTR) gene polymorphism and obsessive compulsive disorder in Chinese Han

ABSTRACT Recent research has shown that genetic variations in the oxytocin receptor (OXTR) may be related to variations in subtypes of obsessive-compulsive disorder (OCD). We aimed to explore the relationship between different subtypes of OCD and the genetic variation between rs1316193 and rs4686301 of the OXTR. In this case-control study, 92 OCD patients and 92 healthy controls were included in the OCD and control groups, respectively. The Y-BOCS scale was used to assess the severity of the OCD symptoms. The fasting peripheral blood samples were collected to extract DNA. rs4686301 and rs13316193 were genotyped using restriction fragment length polymorphism analysis techniques. Whether the gene frequency of the locus and the distribution of allele frequency were related to OCD were further study by TaqMan allele typing. The rs4686301 locus differed significantly between behavior and control groups. The genotype frequency and allele frequency at the rs4686301 locus were statistically significant between behavior and control groups (P<0.05). There was significant difference in the genotype frequency at the rs13316193 locus between behavior and control groups (P<0.05). The rs4686301 polymorphism of the OXTR may affect the clinical subtype of OCD. The rs13316193 polymorphism of the OXTR may be a risk factor for obsessive-compulsive behavior.

The influence of public physical education curriculum on college students' physical health / A influência da educação física pública sobre a saúde física dos estudantes universitários / Efectos de los programas de educación física pública en la salud física de los estudiantes

ABSTRACT It is particularly important to improve the quality of physical education curriculum and the overall health level of college students. It is difficult to achieve a comprehensive evaluation of the original evaluation method of students' physical health level. Therefore, this study combined the analytic hierarchy process (AHP) and physical fitness training mode to build a university physical health evaluation model. Taking X University as the experimental background, 20 students of different majors in grade 2019 were selected as experimental objects. One group (10 students) adopted the original physical education curriculum mode, and the other group (10 students) integrated physical fitness training into the physical education curriculum. Using the established health evaluation model, the evaluation scores of physical health level of two groups of college students before and after the experiment were comprehensively analyzed. The results showed that the overall score of group A students' physical health level increased; the rising rate was 2.87%. However, there was no significant change in the scores of physical health assessment of group B students. The results show that physical fitness training into the public physical education curriculum can effectively help improve the overall health level of students, and also shows that the introduction of AHP into the evaluation of College Students' physical health level can improve the comprehensiveness of health level analysis. It is hoped that this study can provide some reference for physical education teaching in Colleges and universities, so as to improve the overall teaching level of colleges and universities.

RESUMO É particularmente importante melhorar a qualidade dos programas de educação física e o nível geral de saúde dos estudantes universitários. É difícil obter uma avaliação abrangente do método de avaliação inicial do nível de saúde física dos estudantes. Por conseguinte, este estudo combinou o processo hierárquico analítico (AHP) e o modo de condicionamento físico para construir um modelo universitário de avaliação da saúde física. Tendo a Universidade X como objeto experimental, vinte estudantes de diferentes especializações do período de 2019 foram selecionados como objetos experimentais. Um grupo ( de dez estudantes) adotou o modo de educação física original, e o outro grupo (de dez estudantes) integrou o condicionamento físico no currículo de educação física. Utilizando o modelo estabelecido de avaliação da saúde, as pontuações de avaliação do nível de saúde física de dois grupos de estudantes universitários antes e depois do experimento foram exaustivamente analisadas. Os resultados mostraram que a pontuação global do nível de saúde física de um grupo de estudantes aumentou. A taxa de aumento foi de 2.87%. No entanto, não houve alterações significativas nas pontuações da avaliação para a saúde física dos estudantes do grupo B. Os resultados mostram que o condicionamento físico no currículo público de educação física pode contribuir efetivamente para melhorar o nível geral de saúde dos estudantes, e também mostra que a introdução do processo hierárquico analítico (AHP) na avaliação do nível de saúde física dos estudantes universitários pode melhorar a abrangência da análise do nível de saúde. Espera-se que este estudo possa fornecer uma referência para o ensino de educação física nos colégios e universidades a fim de melhorar o nível de ensino geral das universidades.

RESUMEN Es particularmente importante mejorar la calidad del plan de estudios de educación física y el nivel de salud general de los estudiantes universitarios. Es difícil lograr un análisis integral del método de evaluación original del nivel de salud física de los estudiantes. Por lo tanto, este estudio combinó el proceso de jerarquía analítica (PJA) y el modo de entrenamiento de aptitud física para construir un modelo universitario de evaluación de la salud física. Tomando la Universidad X como base experimental, se seleccionaron como sujetos experimentales 20 estudiantes de diferentes carreras en los cursos de 2019. Un grupo (10 estudiantes) adoptó el modo de plan de estudios de educación física original y el otro grupo (10 estudiantes) integró el entrenamiento de aptitud física en el plan de estudios de educación física. Utilizando el modelo de evaluación de salud establecido, se analizaron exhaustivamente los puntajes de evaluación del nivel de salud física de los dos grupos de estudiantes universitarios antes y después del experimento. Los resultados mostraron que el puntaje general del nivel de salud física de los estudiantes del grupo A aumentó; la tasa de incremento fue del 2,87%. Sin embargo, no hubo cambios significativos en las puntuaciones de la evaluación de la salud física de los estudiantes del grupo B. Los resultados muestran que el entrenamiento de la aptitud física en el plan de estudios público de educación física puede ayudar de manera efectiva a mejorar el nivel de salud general de los estudiantes y también muestra que la introducción de PJA en la evaluación del nivel de salud física de los estudiantes universitarios puede mejorar la precisión del análisis del nivel de salud. Esperamos que este estudio pueda proporcionar una referencia para la enseñanza de la educación física en los colegios y universidades, a fin de mejorar su nivel general en estos establecimientos.

Influence of life satisfaction and parental rearing style on accidental injury of rural primary and middle school students / 中国学校卫生

Objective@#To analyze the impact of life satisfaction and parental rearing methods on accidental injuries of primary and secondary school students in rural areas, and to provide a therotical reference for injury prevention and intervention among primary and secondary school students in rural areas.@*Methods@#A stratified cluster random sampling method was used to select 2 045 rural primary and middle school students in Luliang County, Yunnan Province, questionnaire survey regarding injury, MSLSS and EMBU was administered.@*Results@#The incidence of accidental injury among rural primary and middle school students was 16.19 %, and the rate of boys (19.51%) were higher than that of girls (12.26%) ( χ 2=19.69, P <0.01). Multivariate Logistic regression analysis found that women, low injury risk behavior scores, and high life satisfaction scores were associated with less accidental injuries( OR =0.62, 0.98,0.81), while day to day school and father s overprotection were associated with more for accidental injuries( OR =1.50, 1.04, P <0.05).@*Conclusion@#Parents should pay more attention to children s safety education while creating a good living environment for their children, but they should pay attention to their own education methods to reduce accidental injuries.

Effects of Urtica dioica extract on malignant biological behaviors of breast cancer cells and its possible mechanism / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨大荨麻提取物对乳腺癌细胞增殖、凋亡和细胞周期的影响，并初步探讨其可能的作用机制。方法：用不同质量浓度的大荨麻提取物（0、1、2、4、8、16、32、64 mg/ml）处理乳腺癌细胞MCF-7和MDA-MB-231 24 h，MTT法检测细胞增殖活力，选择中位抑制浓度附近的浓度（5和10 mg/ml）作为给药浓度分别处理MCF-7和MDA-MB-231细胞24 h后，平板克隆形成实验和流式细胞术分别检测大荨麻提取物对乳腺癌细胞增殖、周期和凋亡的影响，WB法检测对细胞周期和凋亡相关蛋白以及PI3K/AKT信号通路相关蛋白表达的影响。在MCF-7细胞用5 mg/ml大荨麻提取物处理的同时转染过表达AKT质粒（大荨麻+AKT组），转染空载质粒为对照组（大荨麻+vec组），WB法检测过表达效率，比较过表达AKT对细胞增殖、周期和凋亡的影响。结果：各大荨麻提取物处理组MCF-7和MDA-MB-231细胞增殖活力均显著低于对照组（P<0.05或P<0.01）；与对照组比较，5或10 mg/ml大荨麻处理组乳腺癌细胞的克隆形成数显著减少，G0/G1期细胞占比和凋亡率显著增加（P<0.05或P<0.01），P21、BAX蛋白表达显著升高而Cyclin D1、CDK4、Bcl2蛋白以及p-PI3K、p-AKT蛋白表达显著降低（P<0.05或P<0.01）。大荨麻+AKT组p-AKT和AKT蛋白表达显著高于大荨麻+vec组，克隆数、S期和G2/M期细胞占比均高于大荨麻+vec组（P<0.05或P<0.01），G0/G1期细胞占比和凋亡率低于大荨麻+vec组（P<0.05或P<0.01）。结论：大荨麻提取物可以抑制乳腺癌细胞增殖、促进凋亡且阻滞细胞在G0/G1期，其作用机制可能与抑制PI3K/AKT信号通路相关。

Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma.

Relationship between parental control and aggressive behavior of middle school students / 中国学校卫生

Objective@#To explore the relationship between parental control and aggressive behavior of middle school students.@*Methods@#Random cluster sampling was used to select 1 046 students.Information was collected through the Chinese version of Parental Control Questionnaire and Buss ＆ Perry Aggression Questionnaire.@*Results@#The score on anger were high among female students and the total score were high among male students(t=6.28,2.56,P<0.01).Multiple comparison showed. the grade two junior middle school students’aggression level is significantly higher than grade three junior middle school students and grade one, two high school students(t=3.22,4.07,3.01,P<0.05). There were significant gender differences in parental control,father’s behavior control and father’s psychological control(P<0.05).Parental control shows a significant grade difference,multiple comparison showed, parental control among junior middle school students is significantly higher than that among high school students(t=3.22, 4.07, 3.01, P<0.05).Parental control and aggressiveness of the students were significantly positively correlated(r=0.16,0.29).There was a significant positive correlation between the psychological control of the parents and the aggressiveness of the boys(r=0.26,0.23).The behavior control and psychological control of the parents were positively correlated with the aggressiveness of the girls(r=0.09,0.13,0.29,0.35)(P<0.05).The Logistic regression analysis showed,father’s psychological control(OR=0.50,95%CI=0.21-0.79) and mother’s psychological control(OR=0.32,95%CI=0.02-0.62) showed significant positive association with aggression of the boys.Mother’s psychological control(OR=0.55,95%CI=0.23-0.87)and parents’ control(OR=0.17,95%CI=0.01-0.33) were positively associated with aggression of the girls(P<0.05).@*Conclusion@#Effective intervention of parental control might help prevent and control aggressive behavior of middle school students.

Associations of aggressive behavior with self-esteem with suicide risk in college students / 中国学校卫生

Objective@#To explore the associations of aggressive behavior with self-esteem with suicide risk in college students.@*Methods@#A total of 4 154 college students in Suzhou, Hefei and Ma’anshan were enrolled in our study by multistage cluster sampling method during October to December 2017. Demographic characteristics, suicide risk, aggression and self-esteem of participants were evaluated by Suicidal Behaviors Questionnaire-Revised (SBQ-R), Buss & Perry Aggression Questionnaire-Chinese Version (AQ-CV) and Rosenberg Self-esteem Questionnaire separately. Aggression and self-esteem were divided into three levels, high (> P 75 ), medium ( P 25 - P 75 ) and low (< P 25 ) level, according to the percentile of the questionnaire score. Multivariate logistic regression model was used to examine the associations of aggression and self-esteem with suicide risk, and to explore gender difference.@*Results@#The gender differences were found in suicide risk, aggressive behavior and self-esteem( χ 2=80.21, 26.47, 26.51 , P <0.01). More girls reported suicide ideation, suicide plan and suicide attempt compared to girls( χ 2=33.03, 25.11, 15.71 , P <0.01). Compared with boys, the detection rate of suicide ideation, suicide plan and suicide attempt of girls were higher( P <0.01). The high aggressive behavior and low self-esteem was correlated with increased risk of suicide, and the interaction effects of aggressive behavior and self-esteem on suicide risk was found. The students with high aggressive behavior and low self-esteem had a higher suicide risk, the multiple adjusted odds ratio was 19.39 (95% CI =11.10-33.87). Gender difference was not found in the associations of aggressive behavior and self-esteem with suicide risk ( P >0.05).@*Conclusion@#Aggressive behavior and self-esteem are correlated with suicide risk in college students.

X-chromosome genetic association test incorporating X-chromosome inactivation and imprinting effects

Studies have shown that many complex diseases are sex-determined. When conducting genetic association studies on Xchromosome, there are two important epigenetic factors which should be considered simultaneously: X-chromosome inactivation and genomic imprinting. Currently, there have been several association tests accounting for the information on X-chromosome inactivation. However, these tests do not take the imprinting effects into account. In this paper, we propose a novel association test simultaneously incorporating X-chromosome inactivation and imprinting effects based on case–parent trios and control–parent trios for female offspring and case–control data for male offspring, denoted by MLRXCII. Extensive simulation studies are carried out to investigate the type I error rate and the test power of the proposed MLRXCII . Simulation results demonstrate that the proposed test controls the type I error rate well andis more powerful than the existing method when imprinting effects exist. The proposed MLRXCII test is valid and powerful in genetic association studies on X-chromosome for qualitative traits and thus is recommended in practice.

Hydrolytic efficiency and isomerization during de-esterification of natural astaxanthin esters by saponification and enzymolysis

Background: Astaxanthin from natural sources is typically esterified with fatty acids; hence, it must be hydrolyzed to remove esters before identification and quantification by conventional HPLC. Alkaline-catalyzed saponification and enzyme-catalyzed enzymolysis are the most commonly used de-esterification methods. However, information on the efficiency and isomerization during de-esterification of natural astaxanthin esters by these two methods remains scarce. Therefore, we conducted two HPLC-based experiments to determine which method is better for hydrolyzing astaxanthin esters. Results: To assess the effect of enzymolysis (0.67 U/mL cholesterol esterase, at 37°C) and saponification (0.021 M NaOH, at 5°C) conditions on free astaxanthin recovery and destruction or structural transformation of astaxanthin, we varied the total treatment time across a range of 195 min. The results showed that enzymolysis and saponification were complete in 60 min and 90 min, respectively. After complete hydrolysis, the maximum free astaxanthin recovery obtained by enzymolysis was 42.6% more than that obtained by saponification. The identification of by-products, semi-astacene and astacene, during the process of saponification also indicated that a more severe degradation of astaxanthin occurred during saponification. Moreover, the composition of astaxanthin isomers during saponification was similar to that of the isomers during enzymolysis between 30 min and 75 min (all-trans:9-cis:13-cis = 21:3:1, approximately) but dramatically changed after 90 min, whereas the composition in the enzymolysis treatment remained relatively stable throughout. Conclusion: Compared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis.